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施海燕

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施海燕

南京农业大学,植物保护学院,副教授

电话:025-84395479

e-mailyanzishy@njau.edu.cn

地址:江苏省南京市,卫岗1号,南京农业大学植物保护学院

教育经历:

2008/09–2014/06,南京农业大学植物保护学院,博士,王鸣华

2001/09–2004/06,浙江大学,农业与生物技术学院,硕士,朱国念

1997/09–2001/06,浙江大学,农业与生物技术学院,学士

工作经历:

2012/01–今,南京农业大学,植物保护学院,副教授

2007/01–2011/12,南京农业大学,植物保护学院,讲师

2004/072006/12,南京农业大学,植物保护学院,助教

研究方向:

农药残留检测及环境行为研究

主持或参加科研项目及人才计划项目情况:

1、国家重点研发计划,2016YFD0200207、经作体系农药沉降行为及减施因素、2016/01-2020/12775万元、参加。

2、国家自然科学基金青年项目,31301692、新烟碱类杀虫剂多标记时间分辨荧光免疫分析研究、2014/01-2016/1222万元、主持。

3、国家自然科学基金面上项目,31471794、烟碱类杀虫剂噬菌体展示多肽竞争物作用机制与定量构效关系研究、2015/01-2018/1280万元、参加。

4、国家自然科学基金青年项目,31301690、氯噻啉重链抗体亲和性和特异性关键氨基酸残基作用机理的研究、2014/01-2016/1225万元、参加。

论文

1.        Haiyan Shi, Enze Sheng, Ming Ma, Xiude Hua, Minghua Wang, Development of an enhanced colloidal gold immunochromatographic assay for detection of imidaclothiz. Chinese Journal of Analytical Chemistry, 2017, 45: 403-408.

2.        Enze Sheng1, Haiyan Shi1, Liangliang Zhou1, Xiude Hua, Lu Feng, Tong Yu, Minghua Wang, Dual-labeled time-resolved fluoroimmunoassay for simultaneous detection of clothianidin and diniconazole in agricultural samples with Eu and Sm as labels, Food Chemistry 2016 192: 525–530. (co-first author)

3.        Haiyan Shi, Enze Sheng, Lu Feng, Liangliang Zhou, Xiude Hua, Minghua Wang, Simultaneous detection of imidacloprid and parathion by the dual-labeled time-resolved fluoroimmunoassay. Environmental Science and Pollution Research 2015, 22: 14882–14890.

4.        Haiyan Shi, Qing Zhang, Liangliang Zhou, Minghua Wang, Determination of Antibody Adsorption Constant with Parathion, Isoprocarb and Competitors by Liquid Chromatography. Chinese Journal of Analytical Chemistry 2014, 42(1): 131–135.

5.        Haiyan Shi, Hongxia Li, Xiude Hua, Zuntao Zheng, Guonian Zhu, Minghua Wang, Characterization of Multihapten Antigens on Antibody Sensitivity and Specificity for Parathion. Analytcal Letter 2014, 47 (16): 2699–2707.

6.        Xu Yan1, Haiyan Shi1, Minghua Wang, Development of an enzyme-linked immunosorbent assay for the simultaneous determination of parathion and imidacloprid, Analytical Methods, 2012, 4 (12): 4053–4057. (co-first author)

7.        Haiyan Shi, Banghong Zhang, Yonghao Ye, Zuntao Zheng, Minghua Wang, Development of an enzyme-linked immunosorbent assay for the pyrethroid fenpropathrin. Food and Agricultural Immunology 2011, 22: 69–76.

8.        Xiaoxue Jiang1, Haiyan Shi1, Ne Wu, Minghua Wang, Development of an enzyme-linked immunosorbent assay for diniconazole in agricultural samples. Food Chemistry 2011, 125: 1385–1389. (co-first author)

9.        Xiude Hua, Yuan Ding, Jiachuan Yang, Ming Ma, Haiyan Shi, Minghua Wang, Direct competitive fluoroimmunoassays for detection of imidaclothiz in environmental and agricultural samples using quantum dots and europium as labels. Science of the Total Environment 2017, 583: 222–227.

10.    Qing Zhang, Haiyan Shi, Beibei Gao, Mingming Tian, Xiude Hua, Minghua Wang, Enantioseparation and determination of chiral phenylpyrazole insecticide ethiprole in agricultural and environmental samples and its enantioselective degradation in soils. Science of the Total Environment 2016, 542: 845–853.

11.    Qing Zhang, Mingming Tian, Beibei Gao, Haiyan Shi, Xiude Hua, Minghua Wang, Enantioseparation and determination of the triticonazole enantiomers in fruits, vegetables, and soil using efficient extraction and clean-up methods. J Chromatography B 2016, 1009-1010: 130–137.

12.    Xiude Hua, Liangliang Zhou, Lu Feng, Yuan Ding, Haiyan Shi, Limin Wang, Shirley J. Gee, Bruce D. Hammock, Minghua Wang, Competitive and noncompetitive phage immunoassays for the determination of benzothiostrobin. Analytica Chimica Acta 2015, 890: 150–156.

13.    Qing Zhang, Xiude Hua, Yu Yang, Wei Yin, Mingming Tian, Haiyan Shi, Minghua Wang, Stereoselective degradation of flutriafol and tebuconazole in grape. Environmental Science and Pollution Research 2015, 22: 4350 – 4358.

14.    Wei Yin, Xiude Hua, Xiaofeng Liu, Haiyan Shi, Shirley J. Gee, Minghua Wang, Bruce D. Hammock, Development of an enzyme-linked immunosorbent assay for thiacloprid in soil and agro-products with phage-displayed peptide. Analytical Biochemistry 2015, 481: 27–32.

15.    Xiude Hua, Xiaofeng Liu, Wei Yin, Yazhong Xia, Qiujun Zhou, Yiwen Lu, Wei Li, Haiyan Shi, Fengquan Liu, Minghua Wang, A sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for the detection of bifenthrin in a chemical soil barrier. Science of the Total Environment 2015, 502: 246–251.

16.    Meiyun Wang, Xiude Hua, Qing Zhang, Yu Yang, Haiyan Shi, Minghua Wang, Enantioselective Degradation of Metalaxyl in Grape, Tomato, and Rice Plants. Chirality 2015, 27: 109–114.

17.    Ming Li, Ming Ma, Xiude Hua, Haiyan Shi, Qiuxia Wang and Minghua Wang, Quantum dots-basedfluoroimmunoassay for the simultaneous detection of clothianidin and thiacloprid in environmental and agricultural samples. RSC Advance 2015, 5: 3039–3044.

18.    Qing Zhang, Xiude Hua, Haiyan Shi, Jisong Liu, Mingming Tian, Minghua Wang, Enantioselective bioactivity, acute toxicity and dissipation in vegetables of the chiral triazole fungicide flutriafol. Journal of Hazardous Materials 2015, 284: 65–72.

19.    Mingming Tian, Qing Zhang, Haiyan Shi, Beibei Gao, Xiude Hua, Minghua Wang, Simultaneous determination of chiral pesticide flufiprole enantiomers in vegetables, fruits, and soil by high-performance liquid chromatography. Analytical and Bioanalytical Chemistry 2015, 407: 3499–3507.

20.    Qing Zhang, Liangliang Zhou, Yu Yang, Xiude Hua, Haiyan Shi, Minghua Wang, Study on the stereoselective degradation of three triazole fungicides in sediment. Ecotoxicology and Environmental Safety 2015, 117: 1–6.

21.    Lujing Cong, Jing Guo, Jisong Liu, Haiyan Shi, Minghua Wang, Rapid degradation of endosulfan by zero-valent zinc in water and soil. Journal of Environmental Managent 2015, 150:451–455.

22.    Xiude Hua, Wei Yin, Haiyan Shi, Ming Li, Yanru Wang, Hong Wang, Yonghao Ye, Hee Joo Kim, Shirley J. Gee, Minghua Wang, Fengquan Liu, Bruce D. Hammock, Development of phage immuno-loop-mediated isothermal amplification assays for organophosphorus pesticides in agroproducts. Analytical Chemistry 2014, 86: 8441−8447.

23.    Xiude Hua, Xiaofeng Liu, Haiyan Shi, Yanru Wang, Hee Joo Kim, Shirley J. Gee, Minghua Wang, Fengquan Liu, Bruce D. Hammock, Development of a heterologous enzyme-linked immunosorbent assay for organophosphorus pesticides with phage-borne peptide. RSC Advance 2014, 4: 42445–42453.

24.    Yulong Yuan, Xiude Hua, Ming Li, Wei Yin, Haiyan Shi, Minghua Wang, Development of a sensitive indirect competitive enzyme-linked immunosorbent assay based on the monoclonal antibody for the detection of benzothiostrobin residue. RSC Advance 2014, 4: 24406–24411.

25.    Qing Zhang, Mingming Tian, Meiyun Wang, Haiyan Shi, Minghua Wang, Simultaneous enantioselective determination of triazole fungicide Flutriafol in vegetables, fruits, wheat, soil and water by reversed-phase high-performance liquid chromatography. Journal of Agricultural and Food Chemistry, 2014, 62: 2809−2815.

26.    Xu Yan, Haiyan Shi, Minghua Wang, Development of an enzyme-linked immunosorbent assay for the simultaneous determination of parathion and imidacloprid. Analytical Methods 2012, 4 (12), 4053–4057.

专利

1、华修德,王鸣华,刘凤权,施海燕,噬菌体免疫环介导等温扩增检测法,ZL201410272493.4。2、王鸣华;袁玉龙;华修德;施海燕,苯噻菌酯半抗原、人工抗原、特异抗体制备方法及其用途,ZL201310667544.9。

3、王鸣华;施海燕;李波,联苯菊酯抗原、抗体及其应用,ZL200710133285.6。

4、王鸣华,华修德,冯璐,施海燕,苯噻菌酯抗体特异性结合的多肽及其用途,201510418522.8。

5、王鸣华;华修德;殷维;施海燕,一种抗噻虫啉单克隆抗体及其用途,201510174884.7。

6、王鸣华;李明;施海燕,噻虫胺抗原、抗体及其应用,201210363827.X。

教学

1、农药加工与管理

2、农药残留与农产品安全