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华修德

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华修德

南京农业大学,植物保护学院,副教授,江苏高校“青蓝工程”优秀青年骨干教师

电话:025-84395479

e-mailhuaxiude@njau.edu.cn

地址:江苏省南京市,卫岗1号,南京农业大学植物保护学院

教育经历:

2012/02–2012/08,美国加州大学戴维斯分校农,业与环境科学学院,访问学者,Bruce D. Hammock

2007/09–2012/12,南京农业大学植物保护学院,博士刘凤权

2002/09–2006/06,天津农学院,园艺系,学士

工作经历:

2013/02–2014/12南京农业大学,植物保护学院,讲师

2015/01-今,南京农业大学,植物保护学院,副教授

研究方向:

研制农药半抗原、抗体(多克隆抗体、单克隆抗体、纳米抗体)、模拟表位、抗免疫复合体;研发新颖的农药残留免疫分析方法。

主持或参加科研项目及人才计划项目情况:

1、国家重点研发计划,2016YFD0200207、经作体系农药沉降行为及减施因素、2016/01-2020/12775万元、参加。

2、国家自然科学基金面上项目,31471794、烟碱类杀虫剂噬菌体展示多肽竞争物作用机制与定量构效关系研究、2015/01-2018/1280万元、参加。

3、国家自然科学基金青年项目,31301690、氯噻啉重链抗体亲和性和特异性关键氨基酸残基作用机理的研究、2014/01-2016/1225万元、主持。

4、国家自然科学基金青年项目,31301692、新烟碱类杀虫剂多标记时间分辨荧光免疫分析研究、2014/01-2016/1222万元、参加。

5、教育部高等学校博士点基金课题,20130097120006、氯噻啉重链抗体的体外进化及关键氨基酸残基功能的研究、2014/01-2016/124万元、主持。

6、南京农业大学青年科技创新基金(中央高校基本业务费),KJ2013008、新烟碱类杀虫剂特异性噬菌体展示重链抗体筛选的研究、2013/06-2015/065万元、主持。

7、横向课题,2013320001011402、土壤化学屏障中联苯菊酯快速检测技术的研究、2013/06-2015/06、主持。

论文

1.      Hongjie You1, Xiude Hua1, *, Lu Feng, Nana Sun, Qi Rui, Limin Wang, Minghua Wang, Competitive immunoassay for imidaclothiz using upconversion nanoparticles and gold nanoparticles as labels. Microchim Acta 2017, 184: 1085–1092. (co-first author)

2.      Xiude Hua, Yuan Ding, Jiachuan Yang, Ming Ma, Haiyan Shi, Minghua Wang*, Direct competitive fluoroimmunoassays for detection of imidaclothiz in environmental and agricultural samples using quantum dots and europium as labels. Science of the Total Environment 2017, 583: 222–227.

3.      Xiude Hua, Liangliang Zhou, Lu Feng, Yuan Ding, Haiyan Shi, Limin Wang, Shirley J. Gee, Bruce D. Hammock, Minghua Wang, Competitive and noncompetitive phage immunoassays for the determination of benzothiostrobin. Analytica Chimica Acta 2015, 890: 150–156.

4.      Qingkui Fang1, Limin Wang1, Xiude Hua1, Yulong Wang, Suyan Wang, Qi Cheng, Jia Cai, Fengquan Liu, An enzyme-linked chemiluminescent immunoassay developed for detection of Butocarboxim from agricultural products based on monoclonal antibody. Food Chemistry 2015, 166: 372–379. (co-first author)

5.      Xiude Hua, Xiaofeng Liu, Wei Yin, Yazhong Xia, Qiujun Zhou,Yiwen Lu,Wei Li, Haiyan Shi, Fengquan Liu, Minghua Wang, A sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for the detection of bifenthrin in a chemical soil barrier. Science of the Total Environment 2015, 502: 246–251.

6.      Meiyun Wang1, Xiude Hua1, Qing Zhang, Yu Yang, Haiyan Shi, Minghua Wang, Enantioselective Degradation of Metalaxyl in Grape, Tomato, and Rice Plants. Chirality 2015, 27: 109–114. (co-first author)

7.      Baiping Zhao1, Xiude Hua1, Fei Wang1, Weiliang Dong, Zhoukun Li, Yu Yang, Zhongli Cui, Minghua Wang, Biodegradation of propyzamide by Comamonas testosterone W1 and cloning of the propyzamide hydrolase gene camH. Bioresource Technology 2015, 179: 144–149. (co-first author)

8.      Qing Zhang1, Xiude Hua1, Yu Yang, Wei Yin, Mingming Tian, Haiyan Shi, Minghua Wang, Stereoselective degradation of flutriafol and tebuconazole in grape. Environmental Science and Pollution Research 2015, 22: 4350 – 4358. (co-first author)

9.      Wei Yin1, Xiude Hua1, Xiaofeng Liu, Haiyan Shi, Shirley J. Gee, Minghua Wang, Bruce D. Hammock, Development of an enzyme-linked immunosorbent assay for thiacloprid in soil and agro-products with phage-displayed peptide. Analytical Biochemistry 2015, 481: 27–32. (co-first author)

10.  Xiude Hua, Wei Yin, Haiyan Shi, Ming Li, Yanru Wang, Hong Wang, Yonghao Ye, Hee Joo Kim, Shirley J. Gee, Minghua Wang, Fengquan Liu, Bruce D. Hammock, Development of phage immuno-loop-mediated isothermal amplification assays for organophosphorus pesticides in agroproducts. Analytical Chemistry 2014, 86: 8441−8447.

11.  Ming Li1, Xiude Hua1, Ming Ma, Jisong Liu, Liangliang Zhou, Minghua Wang, Detecting clothianidin residues in environmental and agricultural samples using rapid, sensitive enzyme-linked immunosorbent assay and gold immunochromatographic assay. Science of the Total Environment 2014, 499: 1–6. (co-first author)

12.  Xiude Hua, Xiaofeng Liu, Haiyan Shi, Yanru Wang, Hee Joo Kim, Shirley J. Gee, Minghua Wang, Fengquan Liu, Bruce D. Hammock, Development of a heterologous enzyme-linked immunosorbent assay for organophosphorus pesticides with phage-borne peptide. RSC Advance 2014, 4: 42445–42453.

13.  Yulong Yuan1, Xiude Hua1, Ming Li, Wei Yin, Haiyan Shi, Minghua Wang, Development of a sensitive indirect competitive enzyme-linked immunosorbent assay based on the monoclonal antibody for the detection of benzothiostrobin residue. RSC Advance 2014, 4: 24406–24411. (co-first author)

14.  Xiude Hua, Limin Wang, Gang Li, Qinkui Fang, Fengquan Liu. Multi-analyte enzyme-linked immunosorbent assay for organophosphorus pesticides and neonicotinoid insecticides using a bispecific monoclonal antibody. Analytical Methods 2013, 5: 1556–1563.

15.  Xiude Hua, Jifei Yang, Limin Wang, Qingkui Fang, Gaiping Zhang, Fengquan Liu. Development of an immunochromatographic assay for the rapid detection of OP pesticides in different agricultural products. PLoS one 2012, 7: e53099.

16.  Xiude Hua, Guoliang Qian, Jifei Yang, Baishi Hu, Jiaqin Fan, Na Qin, Gang Li, Yuyan Wang, Fengquan Liu. Development of an immunochromatographic assay for the rapid detection of chlorpyrifos-methyl in water samples. Biosensors and Bioelectronics 2010, 26: 189–194.

17.  Haiyan Shi, Enze Sheng, Ming Ma, Xiude Hua, Minghua Wang, Development of an enhanced colloidal gold immunochromatographic assay for detection of imidaclothiz. Chinese Journal of Analytical Chemistry, 2017, 45: 403-408.

18.  Mingming Tian , Qing Zhang, Xiude Hua, Bowen Tang, Beibei Gao, Minghua Wang, Systemic stereoselectivity study of flufiprole: Stereoselective bioactivity, acute toxicity and environmental fate. Journal of Hazardous Materials 2016, 320: 487–494.

19.  Enze Sheng, Haiyan Shi, Liangliang Zhou, Xiude Hua, Lu Feng, Tong Yu, Minghua Wang, Dual-labeled time-resolved fluoroimmunoassay for simultaneous detection of clothianidin and diniconazole in agricultural samples. Food Chemistry 2016 192: 525–530.

20.  Qing Zhang, Haiyan Shi, Beibei Gao, Mingming Tian, Xiude Hua, Minghua Wang, Enantioseparation and determination of chiral phenylpyrazole insecticide ethiprole in agricultural and environmental samples and its enantioselective degradation in soils. Science of the Total Environment 2016, 542: 845–853.

21.  Qing Zhang, Mingming Tian, Beibei Gao, Haiyan Shi, Xiude Hua, Minghua Wang, Enantioseparation and determination of the triticonazole enantiomers in fruits, vegetables, and soil using efficient extraction and clean-up methods. J Chromatography B 2016, 1009-1010: 130–137.

22.  Ming Ma, Mo Chen, Lu Feng, Hongjie You, Rui Yang, Anna Boroduleva, Xiude Hua, Sergei A. Eremin, Minghua Wang, Fluorescence Polarization Immunoassay for Highly Efficient Detection of Imidaclothiz in Agricultural Samples. Food Anal. Methods 2016, 9:2471–2478.

23.  Ming Li, Ming Ma, Xiude Hua, Haiyan Shi, Qiuxia Wang and Minghua Wang, Quantum dots-basedfluoroimmunoassay for the simultaneous detection of clothianidin and thiacloprid in environmental and agricultural samples. RSC Advance 2015, 5: 3039–3044.

24.  Qing Zhang, Xiude Hua, Haiyan Shi, Jisong Liu, Mingming Tian, Minghua Wang, Enantioselective bioactivity, acute toxicity and dissipation in vegetables of the chiral triazole fungicide flutriafol. Journal of Hazardous Materials 2015, 284: 65–72.

25.  Mingming Tian, Qing Zhang, Haiyan Shi, Beibei Gao, Xiude Hua, Minghua Wang, Simultaneous determination of chiral pesticide flufiprole enantiomers in vegetables, fruits, and soil by high-performance liquid chromatography. Analytical and Bioanalytical Chemistry 2015, 407: 3499–3507.

26.  Qing Zhang, Liangliang Zhou, Yu Yang, Xiude Hua, Haiyan Shi, Minghua Wang, Study on the stereoselective degradation of three triazole fungicides in sediment. Ecotoxicology and Environmental Safety 2015, 117: 1–6.

27.  Ming Li, Enze Sheng, Yulong Yuan, Xiaofeng liu, Xiude Hua, Minghua Wang, Sensitive time-resolved fluoroimmunoassay for quantitative determination clothianidin in agricultural samples. Environmental Science and Pollution Research 2014, 21 (9): 5803–5809.

28.  Ming Li, Xiaofeng Liu, Xiude Hua, Wei Yin, Qingkui Fang and Minghua Wang, Fluorescence polarization immunoassay for highly efficient detection of clothianidin in agricultural samples. Analytical Methods 2014, 6: 6541–6547.

29.  Haiyan Shi, Hongxia Li, Xiude Hua, Zuntao Zheng, Guonian Zhu, Minghua Wang, Characterization of Multihapten Antigens on Antibody Sensitivity and Specificity for Parathion. Analytcal Letter 2014, 47 (16): 2699–2707.

30.  Zhenjiang Liu, Xu Yan, Xiude Hua, Minghua Wang, Time-resolved fluoroimmunoassay for quantitative determination of thiacloprid in agricultural samples. Analytical Methods 2013, 5: 3572–3576.

专利

1.        华修德,王鸣华,刘凤权,施海燕,噬菌体免疫环介导等温扩增检测法,ZL201410272493.4

2.        王鸣华,赵百萍,华修德,一株可有效降解炔苯酰草胺的降解菌及其应用,ZL201410017230.9

3.        王鸣华,袁玉龙,华修德,施海燕,苯噻菌酯半抗原、人工抗原、特异抗体制备方法及其用途,ZL201310667544.9

4.        刘凤权,王利民 ,华修德, 李刚,一种同时检测吡虫啉和甲基对硫磷的双特异单克隆抗体,ZL201210238321.6

5.        刘凤权,王利民, 华修德,有机磷农药多残留快速免疫金标试纸条及其制备方法,ZL201210237844.9

6.        刘凤权,王利民,秦娜,方庆奎,华修德,抗三种有机磷农药的单克隆抗体及其应用ZL201310365944.4

7.        刘凤权,王利民,方庆奎,华修德,氨基甲酸肟酯类农药丁酮威单克隆抗体的制备方法,ZL201310134272.6

8.        王鸣华,华修德,丁园, 氯噻啉抗体特异性结合的噬菌体展示多肽及其用途, 201611271306.6

9.        华修德,王鸣华,周亮亮,丁园,苯噻菌酯免疫复合体特异性结合的多肽及其用途,201510418524.7

10.    王鸣华,华修德,冯璐,施海燕,苯噻菌酯抗体特异性结合的多肽及其用途,201510418522.8

11.    陆艺文,王鸣华,夏亚中,华修德,周秋君,李伟,抗联苯菊酯单克隆抗体及其用途,201410359926X

12.    王鸣华,华修德,殷维,施海燕,一种抗噻虫啉单克隆抗体及其用途,201510174884.7

13.    刘凤权,华修德,钱国良,秦娜,李刚,快速检测甲基毒死蜱的免疫胶体金试纸条及其制备方法201010204947.6

14.    刘凤权,李刚,钱国良,华修德,秦娜,一种应用于吡虫啉农药残留检测的单克隆抗体,201010204933.4

教学

植物化学保护实验

农药残留与农产品安全

奖励

华修德(2/11),农产品与环境中农药、重金属快速检测技术与产品,中国植物保护学会,科学技术奖,一等奖,2016

华修德 老师

个人简历

华修德, 安徽宁国人,博士,农药科学系副教授。 2002/09–2006/06,天津农学院园艺系,农学学士。 2007/09–2012/12,南京农业大学植物保护学院,农学博士; 2012/02–2012/08,美国加州大学戴维斯分校农业与环境科学学院,访问学者; 2013/02–今,南京农业大学,植物保护学院农药科学系任教。 

研究方向

农药残留检测及环境毒理 

研究成果

(1) Xiude Hua, Guoliang Qian, Jifei Yang, Baishi Hu, Jiaqin Fan, Na Qin, Gang Li, Yuyan Wang, Fengquan Liu. Development of an immunochromatographic assay for the rapid detection of chlorpyrifos-methyl in water samples. Biosensors and Bioelectronics, 26: 189-194, 2010. (2) Xiude Hua, Jifei Yang, Limin Wang, Qingkui Fang, Gaiping Zhang, Fengquan Liu. Development of an immunochromatographic assay for the rapid detection of OP pesticides in different agricultural products. PLoS one, 7: e53099, 2012. (3) Xiude Hua, Limin Wang, Gang Li, Qinkui Fang, Fengquan Liu. Multi-analyte enzyme-linked immunosorbent assay for organophosphorus pesticides and neonicotinoid insecticides using a bispecific monoclonal antibody. Analytical Methods, 5, 1556-1563, 2013. (4) Zhenjiang Liu,Xu Yan, Xiude Hua,Minghua Wang. Time-resolved fluoroimmunoassay for quantitative determination of thiacloprid in agricultural samples. Analytical Methods,5, 3572-3576, 2013. 

联系方式

Tel:025-84395479 e-mail:huaxiude@njau.edu.cn