吴益东 老师

个人简历

吴益东，教授，博士生导师。1994年获得南京农业大学昆虫专业博士学位，2000年晋升教授。先后在英国Rothamsted研究所、法国CIRAD和澳大利亚墨尔本大学遗传系进行2年合作研究。目前担任Pest Management Science副编辑、Insect Science和Journal of Integrative Agriculture编委。

长期从事农业害虫抗药性研究，主持和承担国家自然科学基金、863、转基因生物品种培育重大科研专项、行业科技专项等课题20多项。在Nature Biotechnology, PNAS, Nature Communications, Evolutionary Applications, Insect Biochemistry and Molecular Biology, Applied and Environmental Microbiology, Pest Management Science, Pesticide Biochemistry and Physiology等重要国际学术刊物发表SCI论文70余篇。

获教育部科技进步奖二等奖1项（1999，第1完成人）、教育部自然科学奖二等奖1项（2006，第2完成人）、国家科学技术进步奖二等奖1项（2010，第3完成人）。先后获得教育部首届高校青年教师奖（1999）、江苏省第六届青年科技奖（2000）、江苏省"333高层次人才培养工程"首批中青年科技领军人才（2007）、“新世纪百千万人才工程”国家级人选（2009）、全国农业科研杰出人才（2015）。                            

讲授课程

昆虫毒理学（研究生课程）

研究方向

昆虫分子毒理，害虫抗药性，Bt作物抗性监测与治理

研究课题

* 棉铃虫对Bt毒素Cry1Ac和Cry2Ab交互抗性的遗传多样性及机理，国家自然科学基金重点项目，2016-2020.

* 棉铃虫对抗虫棉花的抗性监测技术与治理对策，转基因生物新品种培育重大专项，2016-2020.

* 棉铃虫钙粘蛋白胞内和胞外结构域在Bt 毒素Cry1Ac毒杀过程中的作用，国家自然科学基金面上项目，2013-2016.

* 重要鳞翅目害虫抗药性监测及治理技术研究与示范，公益性（农业）行业科研专项经费项目，2012-2016.

* 小菜蛾抗药性监测与机理，公益性（农业）行业科研专项经费项目，2011-2015.

* 棉铃虫对Bt棉花抗性频率监测与风险管理,转基因生物新品种培育重大专项，2011-2015.

* 棉铃虫Cry1Ac抗性显性遗传品系的抗性机理及适合度，国家自然科学基金面上项目，2011-2013.

* Bt毒素Cry1Ac与棉铃虫中肠受体钙粘蛋白和氨肽酶的互作，国家自然科学基金面上项目，2009-2011.

* 棉铃虫对Bt毒素抗性的高通量分子检测技术，863项目，2007-2010.

* 杀虫药剂诱导害虫再猖獗的机制，973项目子课题骨干，2006-2010.

* 棉铃虫田间种群钙粘蛋白基因变异与对Bt毒素抗性的关系，国家自然科学基金面上项目，2006-2008.

* 用RNA干涉技术研究棉铃虫细胞色素P450基因与抗药性的关系，国家自然科学基金面上项目，2005-2007.

* 小规模棉花生产体系棉铃虫的可持续控制，国际合作项目(CFC/ICAC/14)，2000-2005.

* BT棉在中国的农业和环境适合性评估，欧盟第五框架国际合作项目，2001-2005.

* 棉铃虫细胞色素P450同工酶的纯化及抗性基因检测，国家自然科学基金项目，1998-2000.

* 棉铃虫细胞色素P450与抗药性的关系，国家自然科学基金青年基金项目，1995-1997.

研究成果

(*通讯作者)

2018：

Jin, L., Wang J., Guan F., Zhang J.P., Yu S., Liu S.Y., Xue Y.Y., Li, L.L., Wu S.W., Wang X.L., Yang Y.H., Abdelgaffar H., Jurat-Fuentes J.L., Tabashnik B.E., and Wu Y.D.* 2018. Dominant point mutation in a tetraspanin gene associated with field-evolved resistance of cotton bollworm to transgenic Bt cotton. Proc Natl Acad Sci USA 115(46): 11760-11765.

Wang, H.D., Shi Y., Wang L., Liu S., Wu S.W., Yang Y.H., Feyereisen R., and Wu Y.D.* 2018. CYP6AE gene cluster knockout in Helicoverpa armigera reveals role in detoxification of phytochemicals and insecticides. Nature Communications 9: 4820.

Gao, M.J., Wang X.M., Yang Y.H., Tabashnik B.E., and Wu Y.D.* 2018. Epistasis confers resistance to Bt toxin Cry1Ac in the cotton bollworm. Evolutionary Applications 11(5): 809-819.

Shi, Y., Wang H.D., Liu Z., Wu S.W., Yang Y.H., Feyereisen R., Heckel D.G., and Wu Y.D.* 2018. Phylogenetic and functional characterization of ten P450 genes from the CYP6AE subfamily of Helicoverpa armigera involved in xenobiotic metabolism. Insect Biochemistry and Molecular Biology 93: 79-91.

Zuo Y.Y., Huang J.L., Wang J., Feng Y., Han T.T., Wu Y.D., Yang Y.H.* 2018. Knockout of a P-glycoprotein gene increases susceptibility to abamectin and emamectin benzoate in Spodoptera exigua. Insect Molecular Biology 27(1): 36-45.

Wang, H.D., Zhang J.P., Zhao S., Zhu K.Y., and Wu Y.D.* 2018. Limited variations in susceptibility to an insecticidal double-stranded RNA (dsvATPaseE ) among a laboratory strain and seven genetically differentiated field populations of Tribolium castaneum. Pesticide Biochemistry and Physiology 149: 143-148.

2017：

Zhang, H.N., Yu S., Shi Y., Yang Y.H., Fabrick J.A., and Wu Y.D.* 2017. Intra- and extracellular domains of the Helicoverpa armigera cadherin mediate Cry1Ac cytotoxicity. Insect Biochemistry and Molecular Biology 86:41-49.

Wang, X.L., Puinean A.M., O’Reilly O.R., Williamson M.S., Smelt C.L.C., Millar N.S., and Wu Y.D.* 2017. Mutations on M3 helix of Plutella xylostella glutamate-gated chloride channel confer unequal resistance to abamectin by two different mechanisms. Insect Biochemistry and Molecular Biology 86: 50-57.

Wang, J., Wang H.D., Liu S.Y., Liu L.P., Tay W.T., Walsh T.K., Yang Y.H., and Wu Y.D.* 2017. CRISPR/Cas9 mediated genome editing of Helicoverpa armigera with mutations of an ABC transporter gene HaABCA2 confers resistance to Bacillus thuringiensis Cry2A toxins. Insect Biochemistry and Molecular Biology 87:147-153.

Wei, Y.Y., Wu S.W., Yang Y.H., and Wu Y.D.* 2017. Baseline susceptibility of field populations of Helicoverpa armigera to Bacillus thuringiensis Vip3Aa toxin and lack of cross-resistance between Vip3Aa and Cry toxins. Toxins 9:127.

Zuo Y.Y., Wang H., Xu Y.J., Huang J.L., Wu S.W., Wu Y.D., Yang Y.H.* 2017. CRISPR/Cas9 mediated G4946E substitution in the ryanodine receptor of Spodoptera exigua confers high levels of resistance to diamide insecticides. Insect Biochemistry and Molecular Biology 89: 79-85.

Liu L.P., Gao M.J., Yang S., Liu S.Y., Wu Y.D., Carrière Y., and Yang Y.H.* 2017. Resistance to Bacillus thuringiensis toxin Cry2Ab and survival on single-toxin and pyramided cotton in cotton bollworm from China. Evolutionary Applications 10: 170-179.

Pearce, S.L., Clarke D.F., East P.D., Elfekih S., Gordon K.H.J.*, Jermiin L.S., McGaughran A., Oakeshott J.G.*, Papanikolaou A., Perera O.P., Rane R.V., Richards S., Tay W.T., Walsh T.K., Anderson A., Anderson C.J., Asgari S., Board P.G., Bretschneider A., Campbell P.M., Chertemps T., Christeller J.T., Coppin C.W., Downes S.J., Duan G., Farnsworth C.A., Good R.T., Han L.B., Han Y.C., Hatje K., Horne I., Huang Y.P., Hughes D.S.T., Jacquin-Joly E., James W., Jhangiani S., Kollmar M., Kuwar S.S., Li S., Liu N.Y., Maibeche M.T., Miller J.R., Montagne N., Perry T., Qu J., Song S.V., Sutton G.G., Vogel H., Walenz B.P., Xu W., Zhang H.J., Zou Z., Batterham P., Edwards O.R., Feyereisen R., Gibbs R.A., Heckel D.G., McGrath A., Robin C., Scherer S.E., Worley K.C., and Wu Y.D. 2017. Genomic innovations, transcriptional plasticity and gene loss underlying the evolution and divergence of two highly polyphagous and invasive Helicoverpa pest species. BMC Biology 15:63.

Ni, M., Ma W., Wang X.F., Gao M.J., Dai Y., Wei X., Zhang L., Peng Y., Chen S., Ding L., Tian Y., Li J., Wang H., Wang X., Xu G., Guo W.Z., Yang Y.H., Wu Y.D., Heuberger S., Tabashnik B.E.*, Zhang T.Z.*, Zhu Z.* 2017. Next-generation transgenic cotton: pyramiding RNAi and Bt counters insect resistance. Plant Biotechnology Journal 15:1204-1213.

2016：

Wang J., Zhang H.N., Wang H.D., Zhao S., Zuo Y.Y., Yang Y.H., and Wu Y.D.* 2016. Functional validation of cadherin as a receptor of Bt toxin Cry1Ac in Helicoverpa armigera utilizing the CRISPR/Cas9 system. Insect Biochemistry and Molecular Biology 76: 11-17.

Wang J., Wang X.L., Lansdell S.J., Zhang J.H., Millar N.S., and Wu Y.D.* 2016. A three amino acid deletion in the transmembrane domain of the nicotinic acetylcholine receptor α6 subunit confers high-level resistance to spinosad in Plutella xylostella. Insect Biochemistry and Molecular Biology 71: 29-36.

Wang X.L., Wang R., Yang Y.H., Wu S.W., O'Reilly A.O., and Wu Y.D.* 2016. A point mutation in the glutamate-gated chloride channel of Plutella xylostella is associated with resistance to abamectin. Insect Molecular Biology 25(2): 116-125.

Wang X.L., Su W., Zhang J.H., Yang Y.H., Dong K., and Wu Y.D.* 2016. Two novel sodium channel mutations associated with resistance to indoxacarb and metaflumizone in the diamondback moth, Plutella xylostella. Insect Science 23(1): 50-58.

Wang X.L., Wu S.W., Gao W.Y., and Wu Y.D.* 2016. Dominant inheritance of field-evolved resistance to fipronil in Plutella xylostella (Lepidoptera: Plutellidae). Journal of Economic Entomology 109(1): 334-338.

2015：

Jin L., Zhang H.N., Lu Y.H., Yang Y.H., Wu K.M., Tabashnik B.E., and Wu Y.D.* 2015. Large-scale test of the natural refuge strategy for delaying insect resistance to transgenic Bt crops. Nature Biotechnology 33(2): 169-174.

Wu, S.W., Zuo K.R., Kang Z.K., Yang Y.H., Oakeshott J.G., and Wu Y.D.* 2015. A point mutation in the acetylcholinesterase-1 gene is associated with chlorpyrifos resistance in the plant bug Apolygus lucorum. Insect Biochemistry and Molecular Biology 65: 75-82.

Han, Y.C., Yu W.T., Zhang W.Q., Yang Y.H., Walsh T., Oakeshott J.G., and Wu Y.D.* 2015. Variation in P450-mediated fenvalerate resistance levels is not correlated with CYP337B3 genotype in Chinese populations of Helicoverpa armigera. Pesticide Biochemistry and Physiology 121: 129-135.

Che, W.N., Huang J.L., Guan F., Wu Y.D., and Yang Y.H.* 2015. Cross-resistance and inheritance of resistance to emamectin benzoate in Spodoptera exigua (Lepidoptera: Noctuidae). Journal Economic Entomology 108(4): 2015-2020.

Yuan, G.R., Y. Li, Farnsworth C.A., Coppin C.W., Devonshire A.L., Scott C., Russell R.J., Wu Y.D., and Oakeshott J.G.* 2015. Isomer-specific comparisons of the hydrolysis of synthetic pyrethroids and their fluorogenic analogues by esterases from the cotton bollworm Helicoverpa armigera. Pesticide Biochemistry and Physiology 121: 102-106.

Jiang, D.X., Du Y.Z., Nomura Y., Wang X.L., Wu Y.D., Zhorov B.S., and Dong K.* 2015. Mutations in the transmembrane helix S6 of domain IV confer cockroach sodium channel resistance to sodium channel blocker insecticides and local anesthetics. Insect Biochemistry and Molecular Biology 66: 88-95.

Tabashnik, B.E.*, Zhang M., Fabrick J.A., Wu Y.D., Gao M.J., Huang H.N., Wei J.Z., Zhang J., Yelich A., Unnithan G.C., Bravo A., Soberon M., Carriere Y., and Li X.C. 2015. Dual mode of action of Bt proteins: protoxin efficacy against resistant insects. Scientific Reports 5: 15107.

2014：

Wu Y.D.* 2014. Detection and mechanisms of resistance evolved in insects to Cry toxins from Bacillus thuringiensis. Advances in Insect Physiology 47: 297-342.

Zhang H.N., Du B., Yang Y.H.*, Higginson D.M., Carrière Y., and Wu Y.D. 2014. Cadherin mutation linked to resistance to Cry1Ac affects male paternity and sperm competition in Helicoverpa armigera. Journal of Insect Physiology. 70: 67-72.

Wang R., and Wu Y.D.* 2014. Dominant fitness costs of abamectin resistance in Plutella xylostella. Pest Management Science. 70: 1872-1876

2013:

Jin L., Wei Y.Y., Zhang L., Yang Y.H., Tabashnik B.E., and Wu Y.D.* 2013. Dominant resistance to Bt cotton and minor cross-resistance to Bt toxin Cry2Ab in cotton bollworm from China. Evolutionary Applications 6(8): 1222-1235.

Zhang H.N., Tang M.Y., Yang F., Yang Y.H., and Wu Y.D.* 2013. DNA-based screening for an intracellular cadherin mutation conferring non-recessive Cry1Ac resistance in field populations of Helicoverpa armigera. Pesticide Biochemistry and Physiology 107(1): 148-152.

Wang X.L., Khakame S.K., Ye C., Yang Y.H., and Wu Y.D.* 2013. Characterisation of field-evolved resistance to chlorantraniliprole in the diamondback moth, Plutella xylostella, from China. Pest Management Science 69(5): 661-665.

Yang Y.H., Li Y.P., and Wu Y.D.* 2013. Current status of insecticide resistance in Helicoverpa armigera after 15 years of Bt cotton planting in China. Journal of Economic Entomology 106(1): 375-381.

Khakame S.K., Wang X.L., and Wu Y.D.* 2013. Baseline toxicity of metaflumizone and lack of cross resistance between indoxacarb and metaflumizone in diamondback moth (Lepidoptera: Plutellidae). Journal of Economic Entomology 106(3): 1423-1429.

Che W.N., Shi T., Wu Y.D., and Yang Y.H.* 2013. Insecticide resistance status of field populations of Spodoptera exigua (Lepidoptera: Noctuidae) from China. Journal of Economic Entomology 106(4): 1855-1862.

Oakeshott J.G.*, Farnsworth C.A., East P.D., Scott C., Han Y.C., Wu Y.D., and Russell R.J. 2013. How many genetic options for evolving insecticide resistance in heliothine and spodopteran pests? Pest Management Science 69(8): 889-896.

2012：

Zhang H.N., Tian W., Zhao J., Jin L., Yang J., Liu C.H., Yang Y.H., Wu S.W., Wu K.M., Cui J.J., Tabashnik B.E., and Wu Y.D.* 2012. Diverse genetic basis of field-evolved resistance to Bt cotton in cotton bollworm from China. Proc Natl Acad Sci USA 109(26): 10275-10280.

Zhang H.N., Wu S.W., Yang Y.H., Tabashnik B.E., and Wu Y.D.* 2012. Non-recessive Bt toxin resistance conferred by an intracellular cadherin mutation in field-selected populations of cotton bollworm. PLoS ONE 7(12): e53418.

Wang X.L. and Wu Y.D.* 2012. High levels of resistance to chlorantraniliprole evolved in field populations of Plutella xylostella. Journal of Economic Entomology 105(3): 1019-1023.

Wang X.L., Wu S.W., Yang Y.H., and Wu Y.D.* 2012. Molecular cloning, characterization and mRNA expression of a ryanodine receptor gene from diamondback moth, Plutella xylostella. Pesticide Biochemistry and Physiology 102(3): 204-212.

Han Y.C., Wu S.W., Li Y.P., Liu J.W., Campbell P.M., Farnsworth C., Scott C., Russell R.J., Oakeshott J.G., and Wu Y.D.* 2012. Proteomic and molecular analyses of esterases associated with monocrotophos resistance in Helicoverpa armigera. Pesticide Biochemistry and Physiology 104(3): 243-251.

Tabashnik B.E.*, Wu K.M., and Wu Y.D. 2012. Early detection of field-evolved resistance to Bt cotton in China: Cotton bollworm and pink bollworm. Journal of Invertebrate Pathology 110(3): 301-306.

2011：

Zhang H.N., Yin W., Zhao J., Jin L., Yang Y.H., Wu S.W., Tabashnik B.E., and Wu Y.D.* 2011. Early warning of cotton bollworm resistance associated with intensive planting of Bt cotton in China. PLoS ONE 6(8): e22874.

Wu S.W., Yang Y.H., Yuan G.R., Campbell P.M., Teese M.G., Russell R.J., Oakeshott J.G., and Wu Y.D.* 2011. Overexpressed esterases in a fenvalerate resistant strain of the cotton bollworm, Helicoverpa armigera. Insect Biochemistry and Molecular Biology 41(1): 14-21.

Tabashnik B.E.*, Huang F.N., Ghimire M.N., Leonard B.R., Siegfried B.D., Rangasamy M., Yang Y.J., Wu Y.D., Gahan L.J., Heckel D.G., Bravo A., Soberon M. 2011. Efficacy of genetically modified Bt toxins against insects with different genetic mechanisms of resistance. Nature Biotechology 29(12): 1128-1131.

2010：

Wang Z.Y., Yan H.F., Yang Y.H., and Wu Y.D.* 2010. Biotype and insecticide resistance status of the whitefly Bemisia tabaci from China. Pest Management Science 66(12): 1360-1366.

Yuan G.R., Gao W.Y., Yang Y.H., and Wu Y.D.* 2010. Molecular cloning, genomic structure and genetic mapping of two Rdl-orthologous genes of GABA receptors in the diamondback moth, Plutella xylostella. Archives of Insect Biochemistry and Physiology 74(2): 81-90.

Gong Y.J., Wang C.L., Yang Y.H., Wu S.W., and Wu Y.D.* 2010. Characterization of resistance to Bacillus thuringiensis toxin Cry1Ac in Plutella xylostella from China. Journal of Invertebrate Pathology 104(2): 90-96.

Wang X.L., Li X.Y., Shen A.D., and Wu Y.D.* 2010. Baseline susceptibility of the diamondback moth (Lepidoptera: Plutellidae) to chlorantraniliprole in China. Journal of Economic Entomology 103(3): 843-848.

Pu X., Yang Y.H., Wu S.W., and Wu Y.D.* 2010. Characterization of abamectin resistance in a field-evolved multi-resistant population of Plutella xylostella. Pest Management Science 66(4): 371-378.

Zhao J., Jin L., Yang Y.H., and Wu Y.D.* 2010. Diverse cadherin mutations conferring resistance to Bacillus thuringiensis toxin Cry1Ac in Helicoverpa armigera. Insect Biochemistry and Molecular Biology 40(2): 113-118.

Zhang C.Z., Zhang Z.Y., Liu X.J., Jiang W., and Wu Y.D.* 2010. Dissipation and environmental fate of herbicide H-9201 in carrot plantings under field conditions. Food Chemistry 119: 874-879.

2009：

Wang Z.Y., Yao M.D., and Wu Y.D.* 2009. Cross-resistance, inheritance and biochemical mechanisms of imidacloprid resistance in B biotype Bemisia tabaci. Pest Management Science 65(11): 1189-1194.

Wu Y.D.*, Vassal J.M., Royer M., and Pieretti I. 2009. A single linkage group confers dominant resistance to Bacillus thuringiensis δ-endotoxin Cry1Ac in Helicoverpa armigera. Journal of Applied Entomology 133(5): 375-380.

Yang Y.H., Yang Y.J., Gao W.Y., Guo J.J., Wu Y.H., and Wu Y.D.* 2009. Introgression of a disrupted cadherin gene enables susceptible Helicoverpa armigera to obtain resistance to Bacillus thuringiensis toxin Cry1Ac. Bulletin of Entomological Research 99(2): 175-181.

2004-2008：

Yang Y.H., Yue L.N., Chen S., and Wu Y.D.* 2008. Functional expression of Helicoverpa armigera CYP9A12 and CYP9A14 in Saccharomyces cerevisiae. Pesticide Biochemistry and Physiology 92(2): 101-105.

Xu X.J., and Wu Y.D.* 2008. Disruption of Ha_BtR alters binding of Bacillus thuringiensis δ-endotoxin Cry1Ac to midgut BBMVs of Helicoverpa armigera. Journal of Invertebrate Pathology 97(1): 27-32.

Yang Y.J., Chen H.Y., Wu Y.D.*, Yang Y.H., and Wu S.W. 2007. Mutated cadherin alleles from a field population of Helicoverpa armigera confer resistance to Bacillus thuringiensis toxin Cry1Ac. Applied and Environmental Microbiology 73(21): 6939-6944.

Wang L.H., and Wu Y.D.* 2007. Cross-resistance and biochemical mechanisms of abamectin resistance in the B-type Bemisia tabaci. Journal of Applied Entomology 131(2): 98-103.

Yang Y.H., Chen S., Wu S.W., Yue L.N., and Wu Y.D.* 2006. Constitutive overexpression of multiple cytochrome P450 genes associated with pyrethroid resistance in Helicoverpa armigera. Journal of Economic Entomology 99(5): 1784-1789.

Yang Y.J., Chen H.Y., Wu S.W., Yang Y.H., Xu X.J., and Wu Y.D.* 2006. Identification and molecular detection of a deletion mutation responsible for a truncated cadherin of Helicoverpa armigera. Insect Biochemistry and Molecular Biology 36(9): 735-740.

Li A.G., Yang Y.H., Wu S.W., Li C., and Wu Y.D.* 2006. Investigation of resistance mechanisms to fipronil in the diamondback moth (Lepidoptera: Yponomeutidae). Journal of Economic Entomology 99(3): 914-919.

Xu X.J., Yu L.Y., and Wu Y.D.* 2005. Disruption of a cadherin gene associated with resistance to Cry1Ac δ-endotoxin of Bacillus thuringiensis in Helicoverpa armigera. Applied and Environmental Microbiology 71(2): 948-954.

Chen S., Yang Y.H., and Wu Y.D.* 2005. Correlation between fenvalerate resistance and cytochrome P450 mediated O-demethylation activity in Helicoverpa armigera (Lepidoptera: Noctuidae). Journal of Economic Entomology 98(3): 943-946.

Yang E.H., Yang Y.H., Wu S.W., and Wu Y.D.* 2005. Relative contribution of detoxifying enzymes to pyrethroid resistance in a resistant strain of Helicoverpa armigera. Journal of Applied Entomology 129(9): 521-525.

Yang Y.H., Wu Y.D.*, Chen S., Devine G.J., Denholm I., Jewess P., and Moores G.D. 2004. The involvement of microsomal oxidases in pyrethroid resistance in Helicoverpa armigera from Asia. Insect Biochemistry and Molecular Biology 34(8): 763-773.

联系方式

Tel: 025-84396062, e-mail: wyd@njau.edu.cn