王鸣华

南京农业大学，植物保护学院，教授，博士生导师

电话：025-84395479

e-mail：wangmha@njau.edu.cn

地址：江苏省南京市，卫岗1号，南京农业大学植物保护学院，210095

教育经历：

1999/9 - 2004/6，南京农业大学，农药学，博士，导师：蒋木庚

1983/9 - 1986/6，中国农业大学，农药学，硕士，导师：陈馥衡

1979/9 - 1983/6，兰州大学，有机化学，学士

工作经历：

2006/12 – 今，南京农业大学，植物保护学院，教授

2003/3 – 2006/12，南京农业大学，植物保护学院，副教授

1989/12 - 2003/3，南京农业大学，理学院，副教授

1986/7 - 1989/12，南京农业大学，理学院，讲师

研究方向：

1、农药残留免疫分析

2、手性农药环境行为与毒理

3、农药残留降解

主持或参加科研项目及人才计划项目情况：

1、国家重点研发计划，2016YFD0200207、经作体系农药沉降行为及减施因素、2016/01-2020/12、775万元、主持。

2、国家自然科学基金面上项目，31471794、烟碱类杀虫剂噬菌体展示多肽竞争物作用机制与定量构效关系研究、2015/01-2018/12、80万元、主持。

3、公益性行业（农业）科研专项经费项目，201203022、农作物主要病虫害新药剂、新剂型研究与应用、2012/01-2016/12、60万元、参加。

4、“十二五”农村领域国家科技计划课题，2012BAD14B02、循环农业系统污染物减控关键技术研究、 2012/01-2016/12、20万元、参加。

5、国家863项目，2011AA100806、食品加工过程安全控制工程化技术研究及设备开发、2011/01-2015/12、66万元、主持。

6、国家公益性行业科研专项，20080325、外来入侵害虫西花蓟马防控技术研究与示范、2008/01-2010/12、150万元、主持。

7、国家863项目，2006AA10Z447、化学农药多残留免疫检测、2006/01-2008/12、15万、参加。

8、江苏省自然科学基金项目，BK2005095、拟除虫菊酯多残留免疫分析及免疫识别机制的研究、2005/06-2008/7、7.5万、主持。

9、农业部农药残留标准转化项目多项。

10、农业部农药登记残留试验项目多项。

论文（2012至今）

1.      Hongjie You, Xiude Hua*, Lu Feng, Nana Sun, Qi Rui, Limin Wang, Minghua Wang*, Competitive immunoassay for imidaclothiz using upconversion nanoparticles and gold nanoparticles as labels. Microchim Acta 2017, 184: 1085–1092.

2.      Xiude Hua, Yuan Ding, Jiachuan Yang, Ming Ma, Haiyan Shi, Minghua Wang*, Direct competitive fluoroimmunoassays for detection of imidaclothiz in environmental and agricultural samples using quantum dots and europium as labels. Science of the Total Environment 2017, 583: 222–227.

3.      Haiyan Shi, Enze Sheng, Ming Ma, Xiude Hua, Minghua Wang*, Development of an enhanced colloidal gold immunochromatographic assay for detection of imidaclothiz. Chinese Journal of Analytical Chemistry, 2017, 45: 403-408.

4.      Mingming Tian , Qing Zhang, Xiude Hua, Bowen Tang, Beibei Gao, Minghua Wang*, Systemic stereoselectivity study of flufiprole: Stereoselective bioactivity, acute toxicity and environmental fate. Journal of Hazardous Materials 2016, 320: 487–494.

5.      Enze Sheng, Haiyan Shi, Liangliang Zhou, Xiude Hua, Lu Feng, Tong Yu, Minghua Wang*, Dual-labeled time-resolved fluoroimmunoassay for simultaneous detection of clothianidin and diniconazole in agricultural samples. Food Chemistry 2016 192: 525–530.

6.      Qing Zhang, Haiyan Shi, Beibei Gao, Mingming Tian, Xiude Hua, Minghua Wang*, Enantioseparation and determination of chiral phenylpyrazole insecticide ethiprole in agricultural and environmental samples and its enantioselective degradation in soils. Science of the Total Environment 2016, 542: 845–853.

7.      Qing Zhang, Mingming Tian, Beibei Gao, Haiyan Shi, Xiude Hua, Minghua Wang*, Enantioseparation and determination of the triticonazole enantiomers in fruits, vegetables, and soil using efficient extraction and clean-up methods. J Chromatography B 2016, 1009-1010: 130–137.

8.      Gao Beibei, Zhang Qing, Tian Mingming, Zhang Zhaoxian, Wang Minghua*, Enantioselective determination of the chiral pesticide isofenphos-methyl in vegetables, fruits, and soil and its enantioselective degradation in pak choi using HPLC with UV detection. Analytical and Bioanalytical Chemistry 2016, 408: 6719–6727.

9.      Ming Ma, Mo Chen, Lu Feng, Hongjie You, Rui Yang, Anna Boroduleva, Xiude Hua, Sergei A. Eremin, Minghua Wang*, Fluorescence Polarization Immunoassay for Highly Efficient Detection of Imidaclothiz in Agricultural Samples. Food Anal. Methods 2016, 9:2471–2478.

10.  Xiude Hua, Liangliang Zhou, Lu Feng, Yuan Ding, Haiyan Shi, Limin Wang, Shirley J. Gee, Bruce D. Hammock, Minghua Wang*, Competitive and noncompetitive phage immunoassays for the determination of benzothiostrobin. Analytica Chimica Acta 2015, 890: 150–156.

11.  Baiping Zhao, Xiude Hua, Fei Wang, Weiliang Dong, Zhoukun Li, Yu Yang, Zhongli Cui, Minghua Wang*, Biodegradation of propyzamide by Comamonas testosterone W1 and cloning of the propyzamide hydrolase gene camH. Bioresource Technology 2015, 179: 144–149.

12.  Qing Zhang, Xiude Hua, Yu Yang, Wei Yin, Mingming Tian, Haiyan Shi, Minghua Wang*, Stereoselective degradation of flutriafol and tebuconazole in grape. Environmental Science and Pollution Research 2015, 22: 4350 – 4358.

13.  Wei Yin, Xiude Hua, Xiaofeng Liu, Haiyan Shi, Shirley J. Gee, Minghua Wang*, Bruce D. Hammock, Development of an enzyme-linked immunosorbent assay for thiacloprid in soil and agro-products with phage-displayed peptide. Analytical Biochemistry 2015, 481: 27–32.

14.  Xiude Hua, Xiaofeng Liu, Wei Yin, Yazhong Xia, Qiujun Zhou, Yiwen Lu, Wei Li, Haiyan Shi, Fengquan Liu, Minghua Wang*, A sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for the detection of bifenthrin in a chemical soil barrier. Science of the Total Environment 2015, 502: 246–251.

15.  Meiyun Wang, Xiude Hua, Qing Zhang, Yu Yang, Haiyan Shi, Minghua Wang*, Enantioselective Degradation of Metalaxyl in Grape, Tomato, and Rice Plants. Chirality 2015, 27: 109–114.

16.  Ming Li, Ming Ma, Xiude Hua, Haiyan Shi, Qiuxia Wang and Minghua Wang*, Quantum dots-basedfluoroimmunoassay for the simultaneous detection of clothianidin and thiacloprid in environmental and agricultural samples. RSC Advance 2015, 5: 3039–3044.

17.  Qing Zhang, Xiude Hua, Haiyan Shi, Jisong Liu, Mingming Tian, Minghua Wang*, Enantioselective bioactivity, acute toxicity and dissipation in vegetables of the chiral triazole fungicide flutriafol. Journal of Hazardous Materials 2015, 284: 65–72.

18.  Mingming Tian, Qing Zhang, Haiyan Shi, Beibei Gao, Xiude Hua, Minghua Wang*, Simultaneous determination of chiral pesticide flufiprole enantiomers in vegetables, fruits, and soil by high-performance liquid chromatography. Analytical and Bioanalytical Chemistry 2015, 407: 3499–3507.

19.  Qing Zhang, Liangliang Zhou, Yu Yang, Xiude Hua, Haiyan Shi, Minghua Wang*, Study on the stereoselective degradation of three triazole fungicides in sediment. Ecotoxicology and Environmental Safety 2015, 117: 1–6.

20.  Lujing Cong, Jing Guo, Jisong Liu, Haiyan Shi, Minghua Wang*, Rapid degradation of endosulfan by zero-valent zinc in water and soil. Journal of Environmental Managent 2015, 150:451–455.

21.  Xiude Hua, Wei Yin, Haiyan Shi, Ming Li, Yanru Wang, Hong Wang, Yonghao Ye, Hee Joo Kim, Shirley J. Gee, Minghua Wang, Fengquan Liu*, Bruce D. Hammock, Development of phage immuno-loop-mediated isothermal amplification assays for organophosphorus pesticides in agroproducts. Analytical Chemistry 2014, 86: 8441−8447.

22.  Ming Li, Xiude Hua, Ming Ma, Jisong Liu, Liangliang Zhou, Minghua Wang*, Detecting clothianidin residues in environmental and agricultural samples using rapid, sensitive enzyme-linked immunosorbent assay and gold immunochromatographic assay. Science of the Total Environment 2014, 499: 1–6.

23.  Xiude Hua, Xiaofeng Liu, Haiyan Shi, Yanru Wang, Hee Joo Kim, Shirley J. Gee, Minghua Wang*, Fengquan Liu, Bruce D. Hammock, Development of a heterologous enzyme-linked immunosorbent assay for organophosphorus pesticides with phage-borne peptide. RSC Advance 2014, 4: 42445–42453.

24.  Xu Yan, Xiaojian Tang, Hongxia Li, Enze Sheng, Dongdong Yang, Minghua Wang*, Rapid Detection of Four Organophosphorous and neonicotinoid toxicants using bi-enzyme tracer-based competitive enzyme-linked immunosorbent assay. Food Analytical Methods, 2014, 7: 1186–1194.

25.  Yulong Yuan, Xiude Hua, Ming Li, Wei Yin, Haiyan Shi, Minghua Wang*, Development of a sensitive indirect competitive enzyme-linked immunosorbent assay based on the monoclonal antibody for the detection of benzothiostrobin residue. RSC Advance 2014, 4: 24406–24411.

26.  Dongdong Yang, Wei Yin, Lujing Cong, Minghua Wang*, Synthesis and characterization of a molecularly imprinted polymer for preconcentration of clothianidin in environmental samples. Analytcal Letter, 2014, 47(15): 2613–2627.

27.  Meiyun Wang, Qing Zhang, Lujing Cong, Wei Yin, Minghua Wang*,  Enantioselective degradation of metalaxyl in cucumber, cabbage, spinach and pakchoi. Chemosphere 2014, 95: 241–246.

28.  Ming Li, Enze Sheng, Yulong Yuan, Xiaofeng liu, Xiude Hua, Minghua Wang*, Sensitive time-resolved fluoroimmunoassay for quantitative determination clothianidin in agricultural samples. Environmental Science and Pollution Research 2014, 21 (9): 5803–5809.

29.  Qing Zhang, Mingming Tian, Meiyun Wang, Haiyan Shi, Minghua Wang*, Simultaneous enantioselective determination of triazole fungicide Flutriafol in vegetables, fruits, wheat, soil and water by reversed-phase high-performance liquid chromatography. Journal of Agricultural and Food Chemistry, 2014, 62: 2809−2815.

30.  Chengying Lu, Xing Yin, Xiaofeng Liu, Minghua Wang*, Study of the Photodegradation Kinetics and Pathways of Hexaflumuron in Liquid Media. Photochemistry and Photobiology 2014, 90 (6): 1219–1223.

31.  Ming Li, Xiaofeng Liu, Xiude Hua, Wei Yin, Qingkui Fang and Minghua Wang*, Fluorescence polarization immunoassay for highly efficient detection of clothianidin in agricultural samples. Analytical Methods 2014, 6: 6541–6547.

32.  Haiyan Shi, Hongxia Li, Xiude Hua, Zuntao Zheng, Guonian Zhu, Minghua Wang*, Characterization of Multihapten Antigens on Antibody Sensitivity and Specificity for Parathion. Analytcal Letter 2014, 47 (16): 2699–2707.

33.  Juhua Chang, Shaoying Liu, Shengli Zhou, Minghua Wang*, Guonian Zhu, Effects of butachlor on reproduction and hormone levels in adult zebrafish (Danio rerio). Experimental and Toxicologic Pathology 2013, 65 (1-2): 205–209.

34.  Zhenjiang Liu, Ming Li, Haiyan Shi, Minghua Wang*, Development and Evaluation of an Enzyme-Linked Immunosorbent Assay for the Determination of Thiacloprid in Agricultural Samples. Food Analytical Methods 2013, 6 (2): 691–697.

35.  Ming Li, Enze Sheng, Lujing Cong, Minghua Wang*, Development of Immunoassays for Detecting Clothianidin Residue in Agricultural Products. Journal of Agricultural and Food Chemistry 2013, 61: 3619–3623.

36.  Zhenjiang Liu, Xu Yan, Xiuying Xu, Minghua Wang*, Development of chemiluminescence enzyme-linked immunosorbent assay for the simultaneous detection of imidaclothiz and thiacloprid in agricultural samples. Analyst 2013, 138 (11): 3280 – 3286.

37.  Zhenjiang Liu, Xu Yan, Xiude Hua, Minghua Wang*, Time-resolved fluoroimmunoassay for quantitative determination of thiacloprid in agricultural samples. Analytical Methods 2013, 5: 3572–3576.

38.  Kewei Ren, Yahui Jin, Mengmeng Cao, Minghua Wang*, Detection of Aryloxyphenoxypropionate Herbicides by Enzyme-linked Immunosorbent Assay. Analytcal Letter 2012, 45(8): 831–840.

39.  Mengmeng Cao, Ming Li, Xu Yan, Minghua Wang*, Stereoselectivity of an enzyme-linked, immunosorbent assay for S-bioallethrin. Analytical Methods 2012, 4(2): 534–538.

40.  Hu Zhang, Mingrong Qian, Xinquan Wang, Xiangyun Wang, Hao Xu, Qiang Wang, Minghua Wang*, HPLC–MS/MS enantioseparation of triazole fungicides using polysaccharide-based stationary phases. Journal of Separation Science 2012, 35(7): 773–781.

41.  Juhua Chang, Wenjun Gui, Minghua Wang*, Guonian Zhu, Effects of butachlor on estrogen receptor, vitellogenin and P450 aromatase gene expression in the early life stage of zebrafish. Journal of Environmental Science and Health, Part A 2012, 47(11): 1672–1677.

42.  Zhenjiang Liu, Yahui Jin, Minghua Wang*, Determination of diniconazole in agricultural samples by sol-gel immunoaffinity extraction procedure coupled with HPLC and ELISA. PLos One 2012, 7(10): e46926.

43.  Xu Yan, Haiyan Shi, Minghua Wang*, Development of an enzyme-linked immunosorbent assay for the simultaneous determination of parathion and imidacloprid. Analytical Methods 2012, 4 (12), 4053–4057.

44.  Zhang Hu, Qian Mingrong, Wang Xinquan, Wang Xiangyun, Xu Hao, Qi Peipei, Wang Qiang, Wang Minghua*, Analysis of Tebuconazole and Tetraconazole Enantiomers by Chiral HPLC-MS/MS and Application to Measure Enantioselective Degradation in Strawberries. Food Analytical Methods 2012, 5(6): 1342–1348.

著作

1.        植物化学保护实验，北京大学出版社

2.        拟除虫菊酯化学，化学工业出版社

3.        农药营销管理学，中国农业出版社

4.        新杂环农药—杀虫剂，化学工业出版社

5.        新杂环农药—除草剂，化学工业出版社

6.        农药残留分析，中国农业出版社

7.        农药立体化学，化学工业出版社

专利

1.        王鸣华，赵百萍，华修德，一种可高效降解炔苯酰草胺的降解菌及其应用，ZL201410017230.9。

2.        王鸣华；袁玉龙；华修德；施海燕，苯噻菌酯半抗原、人工抗原、特异抗体制备方法及其用途，ZL201310667544.9。

3.        王鸣华；施海燕；李波，联苯菊酯抗原、抗体及其应用，ZL200710133285.6。

4.        华修德，王鸣华，刘凤权，施海燕，噬菌体免疫环介导等温扩增检测法，ZL201410272493.4。

5.        王鸣华，华修德，丁园， 氯噻啉抗体特异性结合的噬菌体展示多肽及其用途， 201611271306.6。

6.        王鸣华，华修德，冯璐，施海燕，苯噻菌酯抗体特异性结合的多肽及其用途，201510418522.8。

7.        华修德，王鸣华，周亮亮，丁园，苯噻菌酯免疫复合体特异性结合的多肽及其用途，201510418524.7。

8.        王鸣华；华修德；殷维；施海燕，一种抗噻虫啉单克隆抗体及其用途，201510174884.7。

9.        王鸣华；李明；施海燕，噻虫胺抗原、抗体及其应用，201210363827.X。

10.    陆艺文；王鸣华；夏亚中；华修德；周秋君；李伟，抗联苯菊酯单克隆抗体及其用途，201410359926X。

教学

1、农药学

2、农药加工与管理

3、农药研究新进展

4、植物保护研究方法

奖励

王鸣华（7/11），农产品与环境中农药、重金属快速检测技术与产品，中国植物保护学会，科学技术奖，一等奖，2016